What we’re reading.

See what the Herophilus team is reading this week across the fields of neuroscience, organoids, drug discovery, machine learning, bioassays, disease models and more.

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20 June 2022 Nature

Daniel Rosebrock, Sneha Arora, Naresh Mutukula, Rotem Volkman, Elzbieta Gralinska, Anastasios Balaskas, Amèlia Aragonés Hernández, René Buschow, Björn Brändl, Franz-Josef Müller, Peter F. Arndt, Martin Vingron & Yechiel Elkabetz

Enhanced cortical neural stem cell identity through short SMAD and WNT inhibition in human cerebral organoids facilitates emergence of outer radial glial cells

20 June 2022 Cell

Joseph M. Replogle, Reuben A. Saunders, Angela N. Pogson, Jeffrey A. Hussmann, Alexander Lenail, Alina Guna, Lauren Mascibroda, Eric J. Wagner, Karen Adelman, Gila Lithwick-Yanai, Nika Iremadze, Florian Oberstrass, Doron Lipson, Jessica L. Bonnar, Marco Jost, Thomas M. Norman, Jonathan S. Weissman

Mapping information-rich genotype-phenotype landscapes with genome-scale Perturb-seq

1 June 2022 Nature

Fabien Vincent, Arsenio Nueda, Jonathan Lee, Monica Schenone, Marco Prunotto & Mark Mercola

Phenotypic drug discovery: recent successes, lessons learned and new directions

1 June 2022 Nature

Adam K. Glaser, Kevin W. Bishop, Lindsey A. Barner, Etsuo A. Susaki, Shimpei I. Kubota, Gan Gao, Robert B. Serafin, Pooja Balaram, Emily Turschak, Philip R. Nicovich, Hoyin Lai, Luciano A. G. Lucas, Yating Yi, Eva K. Nichols, Hongyi Huang, Nicholas P. Reder, Jasmine J. Wilson, Ramya Sivakumar, Elya Shamskhou, Caleb R. Stoltzfus, Xing Wei, Andrew K. Hempton, Marko Pende, Prayag Murawala, Hans-Ulrich Dodt, Takato Imaizumi, Jay Shendure, Brian J. Beliveau, Michael Y. Gerner, Li Xin, Hu Zhao, Lawrence D. True, R. Clay Reid, Jayaram Chandrashekar, Hiroki R. Ueda, Karel Svoboda and Jonathan T. C. Liu

A hybrid open-top light-sheet microscope for versatile multi-scale imaging of cleared tissues

28 April 2022 Cell Stem Cell

Mariko L. Bennett, Hongjun Song, Guo-li Ming

Microglia modulate neurodevelopment in human neuroimmune organoids

28 April 2022 Cell Stem Cell

Galina Popova, Sarah S. Soliman, Chang N. Kim, Matthew G. Keefe, Kelsey M. Hennick, Samhita Jain, Tao Li, Dario Tejera, David Shin, Bryant B. Chhun, Christopher S. McGinnis, Matthew Speir, Zev J. Gartner, Shalin B. Mehta, Maximilian Haeussler, Keith B. Hengen, Richard R. Ransohoff, Xianhua Piao, Tomasz J. Nowakowski

Human microglia states are conserved across experimental models and regulate neural stem cell responses in chimeric organoids

27 April 2022 Neuron

Arjun Bharioke, Martin Munz, Alexandra Brignall, Georg Kosche, Max Ferdinand Eizinger, Nicole Ledergerber, Daniel Hillier, Brigitte Gross-Scherf, Karl-Klaus Conzelmann, Emilie Macé, Botond Roska

General anesthesia globally synchronizes activity selectively in layer 5 cortical pyramidal neurons

25 April 2022 bioRxiv

Cristina Cheroni, Sebastiano Trattaro, Nicolò Caporale, Alejandro López-Tobón, Erika Tenderini, Flavia Troglio, Michele Gabriele, Raul Bardini Bressan, Steven M Pollard, William T Gibson, Giuseppe Testa

Benchmarking brain organoid recapitulation of fetal corticogenesis

18 April 2022 Nature

Chao Wang, Li Fan, Rabia R. Khawaja, Bangyan Liu, Lihong Zhan, Lay Kodama, Marcus Chin, Yaqiao Li, David Le, Yungui Zhou, Carlo Condello, Lea T. Grinberg, William W. Seeley, Bruce L. Miller, Sue-Ann Mok, Jason E. Gestwicki, Ana Maria Cuervo, Wenjie Luo & Li Gan

Microglial NF-κB drives tau spreading and toxicity in a mouse model of tauopathy

13 April 2022 Nature

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